Halothiobacillus hydrothermalis (Durand et al.) Kelly and Wood
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2. Using a single tube of #1901 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #1901 agar slant and/or plate.
5. Incubate the tubes and plate at 37°C for 24 hours.
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Durand P, et al. Isolation and characterization of Thiobacillus hydrothermalis, new species, a mesophilic obligately chemolithotrophic bacterium isolated from a deep-sea hydrothermal vent in Fiji Basin. Arch. Microbiol. 159: 39-44, 1993.
Kelly DP, Wood AP. Reclassification of some species of Thiobacillus to the newly designated genera Acidithiobacillus gen. nov., Halothiobacillus gen. nov. and Thermithiobacillus gen. nov.. Int. J. Syst. Evol. Microbiol. 50: 511-516, 2000. PubMed: 10758854