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Borrelia garinii Baranton et al.


Type strain
Product category
Strain designation
CIP 103362
Type strain
Genome sequenced strain
Isolation source
Tick, Ixodes ricinus
Geographical isolation
Infectious disease research
Vector-borne disease research
Product format
Storage conditions
-80°C or colder
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Biosafety Icon Biosafety Level 2

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
Emerging infectious disease research
Vector borne research

Handling information

Handling procedure
  1. Thaw and aseptically transfer the entire contents of the vial to a tube of fresh #1914 broth (5 to 6mL). Mix well.
  2. Transfer one-tenth of the cell suspension to two or three additional tubes of fresh #1914 broth.
  3. Use several drops of the primary broth tube to inoculate a #260 plate.
  4. Incubate at 37°C for 5-7 days.
Handling notes

Borrelia garinii is a fragile, sensitive organism that must have the appropriate medium for growth.  Rabbit serum is essential for the growth of this organism.  Fresh medium enhances growth.  Medium older than one month should not be used. 

Additional information on this culture is available on the ATCC® web site at


Deposited as
Borrelia garinii Baranton et al.
Chain of custody
ATCC <-- CIP <-- G. Baranton 20047
Type of isolate
Cross references
GenBank D67018 Borrelia garinii DNA for 16S ribosomal RNA
GenBank CP018750 Borreliella garinii strain CIP 103362 plasmid cp26, complete sequence
GenBank CP018754 Borreliella garinii strain CIP 103362 plasmid cp32, complete sequence
GenBank CP018755 Borreliella garinii strain CIP 103362 plasmid cp32, complete sequence
GenBank CP018749 Borreliella garinii strain CIP 103362 plasmid lp28-7 sequence
GenBank CP018745 Borreliella garinii strain CIP 103362 plasmid p_lp54_linear sequence
GenBank CP018746 Borreliella garinii strain CIP 103362 plasmid unnamed1 sequence
GenBank CP018747 Borreliella garinii strain CIP 103362 plasmid unnamed2 sequence
GenBank CP018748 Borreliella garinii strain CIP 103362 plasmid unnamed3 sequence
GenBank CP018751 Borreliella garinii strain CIP 103362 plasmid unnamed4 sequence
GenBank CP018752 Borreliella garinii strain CIP 103362 plasmid unnamed5 sequence
GenBank CP018753 Borreliella garinii strain CIP 103362 plasmid unnamed6 sequence

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.



Curated Citations

Baranton G, et al. Delineation of Borrelia burgdorferi sensu stricto, Borrelia garinii sp. nov., and group VS461 associated with Lyme borreliosis. Int. J. Syst. Bacteriol. 42: 378-383, 1992. PubMed: 1380285

Bontemps-Gallo S, et al. Genomic and phenotypic characterization of Borrelia afzelii BO23 and Borrelia garinii CIP 103362. PLoS One 13(6):e0199641, 2018. PubMed: 29944685

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