Clostridium estertheticum subsp. estertheticum Collins et al.
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2. Under anaerobic conditions, withdraw 0.5 ml of chilled #1053 from a single test tube (5 to 6 ml) and rehydrate the entire vial contents.
3. Aseptically transfer this aliquot back into the broth tube. Additional tubes may be inoculated with 0.5 ml each from the suspension. A slant of #1053 may also be inoculated with 0.2 ml. Streak several blood plates to check for colonial morphology and purity.
4. Incubate tubes under an anaerobic atmosphere at 10oC. Incubate one agar plate anaerobically for colony formation, and one aerobically for aerobic contamination check.
Anaerobic conditions for transfer may be obtained by either of the following:
· Use of an anaerobic gas chamber, or
· Placement of test tubes under a gassing cannula system
hooked to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
· Loose screw caps on test tubes in anaerobic chamber,
· Loose screw caps on test tubes in an activated anaerobic
gas pack jar, or
· Use of sterile butyl rubber stoppers on test tubes so that
an anaerobic gas headspace is retained.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
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Collins MD, et al. Taxonomic studies on a psychrophilic Clostridium from vacuum-packed beef: description of Clostridium estertheticum sp. nov.. FEMS Microbiol. Lett. 75: 235-240, 1992. PubMed: 1383083
Validation list no. 44. Int. J. Syst. Bacteriol. 43: 188-189, 1993.
Spring S, et al. Characterization of novel psychrophilic clostridia from an Antarctic microbial mat: description of Clostridium frigoris sp. nov., Clostridium lacusfryxellense sp. nov., Clostridium bowmanii sp. nov. and Clostridium psychrophilum sp. nov. and reclassification of Clostridium laramiense as Clostridium estertheticum subsp. laramiense subsp. nov.. Int. J. Syst. Evol. Microbiol. 53: 1019-1029, 2003. PubMed: 12892121