Halorubrum vacuolatum (Mwatha and Grant) Kamekura et al.
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2. Using a single tube of #1899 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur pipette and rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate an additional broth tube, slant, and/or plate. Incubate plates in a bag with a moist paper towel.
5. Incubate the tubes and plate at 37oC for 4-7 days.
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Mwatha WE, Grant WD. Natronobacterium vacuolata sp. nov., a haloalkaliphilic archaeon isolated from Lake Magadi, Kenya. Int. J. Syst. Bacteriol. 43: 401-404, 1993.
Kamekura M, et al. Diversity of alkaliphilic halobacteria: proposals for transfer of Natronobacterium vacuolatum, Natronobacterium magadii, and Natronobacterium pharaonis to Halorubrum, Natrialba, and Natronomonas gen. nov., respectively, as Halorubrum vacuolatum comb. nov., Natrialba magadii comb. nov., and Natronomonas pharaonis comb. nov., respectively. Int. J. Syst. Bacteriol. 47: 853-857, 1997. PubMed: 9226918