Johnsonella ignava Moore and Moore
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1. Open vial according to enclosed instructions.
2. Under anaerobic conditions, withdraw 0.5 ml of recommended broth from a single test tube (5 to 6 ml) and rehydrate the entire vial contents.
3. Aseptically transfer this aliquot back into the broth tube. A slant and a pre-reduced blood plate may also be inoculated with 0.1 ml each of the cell suspension. An aerobic blood plate may also be streaked to check for purity.
4. Incubate tubes and plate under anaerobic conditions at 37°C. Incubate blood plate aerobically at 37°C.
5. Within 24 to 48 hours, growth should be evident by turbidity in the broth. No growth should occur on the blood agar plate incubated aerobically.
Anaerobic conditions for transfer may be obtained by either of the following:
· Tubes of media are placed under a gassing cannula system hooked to a source of oxygen free gas.
Anaerobic conditions for incubation may be obtained by any of the following:
Additional information on this culture is available on the ATCC web site at www.atcc.org.
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Moore LV, Moore WE. Oribaculum catoniae gen. nov., sp. nov.; Catonella morbi gen. nov., sp. nov.; Hallella seregens gen. nov., sp. nov.; Johnsonella ignava gen. nov., sp. nov.; and Dialister pneumosintes gen. nov., comb. nov., nom. rev., Anaerobic gram-negative bacilli from the human gingival crevice. Int. J. Syst. Bacteriol. 44: 187-192, 1994. PubMed: 8186083
gingival crevice of healthy human