Fibrobacter succinogenes (Hungate) Montgomery et al.
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Anaerobic conditions for transfer may be obtained by the use of an anaerobic gas chamber or placement of test tubes under a gassing cannula system connected to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
We suggest using cysteine and adding that reducing agent to the medium at least one hour before the medium is to be inoculated.
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Amann RI, et al. Diversity among Fibrobacter isolates: Towards a phylogenetic classification. Syst. Appl. Microbiol. 15: 23-31, 1992.