Neisseria gonorrhoeae (Zopf) Trevisan
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2. Using a single tube of #814 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use 0.2 ml of the suspension to inoculate a #814 slant, and/or 0.1 ml for a plate.
5. Incubate all tubes and plate at 37oC with 5% CO2 for 24 to 48 hours. Assure that screw caps are loose during incubation period.
At 24 hours, colonies are pinpoint in size. At 48 hours, colonies vary in size, are circular, entire, and flat gray. Older colonies assume a fried egg appearance and slightly pit the agar. The increased CO2 is helpful for growth.
Brain Heart Infusion (Difco 237500) can be used to rehydrate vial contents if #814 broth is not available, but it will not support the growth of this strain.
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Evans GL, et al. New selective medium for the isolation of Neisseria gonorrhoeae. J. Clin. Microbiol. 27: 2471-2474, 1989. PubMed: 2509512