Toxoplasma gondii (Nicolle and Manceaux) Nicolle and Manceaux
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Cell Line Maintenance
Transferring the Cell Line
Storage and Culture Initiation of Toxoplasma
Frozen ampules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.
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If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Howe DK, Sibley LD. Toxoplasma gondii: analysis of different laboratory stocks of the RH strain reveals genetic heterogeneity. Exp. Parasitol. 78: 242-245, 1994. PubMed: 7907030
Sibley LD, et al. Generation of a restriction fragment length polymorphism linkage map for Toxoplasma gondii. Genetics 132: 1003-1015, 1992. PubMed: 1360931
Howe DK, Sibley LD. Toxoplasma gondii comprises three clonal lineages: correlation of parasite genotype with human disease. J. Infect. Dis. 172: 1561-1566, 1995. PubMed: 7594717
Sibley LD, Boothroyd JC. Virulent strains of Toxoplasma gondii comprise a single clonal lineage. Nature 359: 82-85, 1992. PubMed: 1355855
Howe DK, et al. Acute virulence in mice is associated with markers on chromosome VIII in Toxoplasma gondii. Infect. Immun. 64: 5193-5198, 1996. PubMed: 8945565