Toxoplasma gondii (Nicolle and Manceaux) Nicolle and Manceaux
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Cell Line Maintenance
Transferring the Cell Line
Storage and Culture Initiation of Toxoplasma
Frozen ampules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.
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Howe DK, Sibley LD. Toxoplasma gondii: analysis of different laboratory stocks of the RH strain reveals genetic heterogeneity. Exp. Parasitol. 78: 242-245, 1994. PubMed: 7907030
Howe DK, et al. Acute virulence in mice is associated with markers on chromosome VIII in Toxoplasma gondii. Infect. Immun. 64: 5193-5198, 1996. PubMed: 8945565
Su C, et al. Recent expansion of Toxoplasma through enhanced oral transmission. Science 299: 414-416, 2003. PubMed: 12532022
L D Sibley, personal communication