Blastocystis hominis Brumpt
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
2. The strain grows at the bottom of the liquid overlay as a dense mass of cells. Carefully introduce a sterile Pasteur pipette aseptically through the liquid overlay-air interface (avoid expulsion of air bubbles) and move the tip of the pipette to the cell mass, aspirate approximately one third of the mass into the pipette. Tighten the cap immediately unless placing the tube directly into an anaerobic jar.
3. Inoculate a fresh tube of reduced ATCC medium 1671. Place the freshly inoculated tube into the anaerobic jar with the caps loosened one full turn, prepare the GasPak, and quickly seal the jar. Incubate at 35°C.
4. Subculture every 2-3 days.
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