Bartonella elizabethae (Daly et al.) Brenner et al.
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2. To obtain a biphasic culture, add 0.4 ml of the suspension to a #260 slant. Add remaining 0.1 ml of the suspension to a #260 plate and streak for isolation..
3. Incubate at 37oC in an atmosphere of 5% CO2. Growth usually occurs in 2 to 3 days.
Colonies on #260 agar are white, entire, glistening, circular, and smooth.
Good growth will be present at the broth-agar interface. Under phase microscopy the organisms appear as small rods, singly or in clumps, and non-motile.
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Validation list no. 46. Int. J. Syst. Bacteriol. 43: 624-625, 1993.
Daly JS, et al. Rochalimaea elizabethae sp. nov. isolated from a patient with endocarditis. J. Clin. Microbiol. 31: 872-881, 1993. PubMed: 7681847
Brenner DJ, et al. Proposals to unify the genera Bartonella and Rochalimaea, with descriptions of Bartonella quintana comb. nov., Bartonella vinsonii comb. nov., Bartonella henselae comb. nov., and Bartonella elizabethae comb. nov., and to remove the family Bartonellaceae from the order Rickettsiales. Int. J. Syst. Bacteriol. 43: 777-786, 1993. PubMed: 8240958
Kordick DL, et al. Bartonella vinsonii subsp. berkhoffii subsp. nov., isolated from dogs; Bartonella vinsonii subsp. vinsonii; and emended description of Bartonella vinsonii. Int. J. Syst. Bacteriol. 46: 704-709, 1996. PubMed: 8782679
human blood of patient with endocarditis, Brighton