Bartonella henselae (Regnery et al.) Brenner et al.
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Colonies on #1814 are small, gray, circular, entire, smooth, low convex, and translucent. Cells are Gram negative rods found mostly in clumps.
This is a slow-growing organism that requires moist conditions for best growth. Growth at the broth/agar interface of the biphasic slant should occur within 7-10 days. The use of FRESH media is of primary importance.
Medium #4 (Tryptic Soy Agar with 5% Defibrinated Rabbit Blood) may also be used. Once good growth is obtained, transfer or freeze the culture. Adding an equal amount of 20% sterile glycerol to pooled broth from several biphasic slants, followed by freezing in liquid nitrogen or “ultra-low temperature” freezer, is recommended.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.
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Welch DF, et al. Rochalimaea henselae sp. nov., a cause of septicemia, bacillary angiomatosis, and parenchymal bacillary peliosis. J. Clin. Microbiol. 30: 275-280, 1992. PubMed: 1537892
Slater LN, et al. A newly recognized fastidious gram-negative pathogen as a cause of fever and bacteremia. N. Engl. J. Med. 323: 1587-1593, 1990. PubMed: 2233947
Dehio C, Meyer M. Maintenance of broad-host-range incompatibility group P and group Q plasmids and transposition of Tn5 in Bartonella henselae following conjugal plasmid transfer from Escherichia coli. J. Bacteriol. 179: 538-540, 1997. PubMed: 8990308
blood of a 31-year-old male with AIDS, Oklahoma City