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Thiothrix eikelboomii Howarth et al.

49788

Product category
Bacteria
Strain designation
AP3
Type strain
Yes
Isolation source
Domestic wastewater activated sludge
Geographical isolation
United States; Pennsylvania; Aliquippa
Product format
Frozen
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Preceptrol
No

Characteristics

Comments
Type 021N

Handling information

Medium
Temperature
20°C
Handling procedure
1. From a sterile stock supply of ATCC® #1820 broth, aseptically transfer 10 ml to a sterile 125 ml flask.

2. Thaw the frozen vial at room temperature.

3. Aaseptically transfer the entire contents of the vial into the 125 ml flask.

4. Incubate flask at 20°C for 4-6 days.

Handling notes
Cells are rods and form multicellular white filaments.  Cell variability is observed throughout the filaments.  Cells tend to form tightly woven mats in broth.  Growth on solid medium is difficult at best and can take 3 to 4 weeks.  Colonies are white and rhizoid in appearance.

          Additional information on this culture is available on the ATCC® web site at www.atcc.org.

       

History

Deposited as
Unidentified bacterium
Depositors
RF Unz
Chain of custody
ATCC <-- RF Unz <-- T.M. Williams
Type of isolate
Wastewater
Cross references
GenBank AB042819 Thiothrix eikelboomii gene for 16S rRNA.
GenBank L79965 Thiothrix eikelboomii ribosomal RNA gene, complete sequence.

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Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

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Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

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References

Curated Citations

Williams TM, Unz RF. Filamentous sulfur bacteria of activated sludge: characterization of Thiothrix, Beggiatoa, and Eikelboom type 021N strains. Appl. Environ. Microbiol. 49: 887-898, 1985. PubMed: 4004221

. . Water Res. 23: 15-22, 1989.

Howarth R, et al. Phylogenetic relationships of filamentous sulfur bacteria (Thiothrix spp. and Eikelboom type 021N bacteria) isolated from wastewater-treatment plants and description of Thiothrix eikelboomii sp. nov., Thiothrix unzii sp. nov., Thiothrix fructosivorans sp. nov. and Thiothrix defluvii sp. nov.. Int. J. Syst. Bacteriol. 49: 1817-1827, 1999. PubMed: 10555365

type strain

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