Thiothrix fructosivorans Howarth et al.
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2. Open the frozen vial and aseptically transfer the contents into the 125 ml flask.
3. Incubate flask at 10°C for 4-6 days.
4. Transfer flask contents to 100 ml of #1820 broth contained in a 250 ml Erlenmeyer flask. Incubate flask without shaking at 10°C.
5. Growth is slow and is evident by accumulation of white filaments within the broth.
6. Transfer to fresh medium at 2 month intervals. A 5% inoculum (5ml of culture to 100 ml fresh medium) is recommended.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
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