Hydrogenophaga taeniospiralis (Lalucat et al.) Willems et al.
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2. From a single tube of #18 broth (5 to 6 ml), withdraw approximately 0.5 to 1 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate #18 agar slant(s) and/or plate(s).
5. Incubate all tubes and plates at 30°C for 48 hours.
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Willems A, et al. Hydrogenophaga, a new genus of hydrogen-oxidizing bacteria that includes Hydrogenophaga flava comb. nov. (Formerly Pseudomonas flava), Hydrogenophaga palleronii (Formerly Pseudomonas palleronii), Hydrogenophaga pseudoflava (Formerly Pseudomonas pseudoflava and "Pseudomonas carboxydoflava"), and Hydrogenophaga taeniospiralis (Formerly Pseudomonas taeniospiralis). Int. J. Syst. Bacteriol. 39: 319-333, 1989.