Oxalophagus oxalicus (Dehning and Schink) Collins et al.
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2. Under anaerobic conditions, withdraw 0.5 ml of #2309 from a single test tube (5 to 6 ml) and rehydrate the vial contents.
3. Aseptically transfer this aliquot back into the broth tube. Additional tubes may be inoculated with 0.5 ml each from the suspension.
4. Incubate tubes under an anaerobic atmosphere at 30oC. Incubate one agar plate anaerobically and one aerobically to check for contamination.
Anaerobic conditions for transfer may be obtained by either of the following:
· Use of an anaerobic gas chamber, or
· Placement of test tubes under a gassing cannula system hooked to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
· Loose screw caps on test tubes in anaerobic chamber,
· Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
· Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
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Collins MD, et al. The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int. J. Syst. Bacteriol. 44: 812-826, 1994. PubMed: 7981107
Dehning I, Schink B. Two new species of anaerobic oxalate-fermenting bacteria, Oxalobacter vibrioformis sp. nov. and Clostridium oxalicum sp. nov. from sediment samples. Arch. Microbiol. 153: 79-84, 1989.
freshwater sediment, Tubingen; November; I. Dehning