Prevotella dentalis (Haapasalo et al.) Willems and Collins
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2. Under anaerobic conditions, withdraw 0.5 ml of the recommended broth from a single test tube (5 to 6 ml) and rehydrate the entire vial contents.
3. Aseptically transfer this aliquot back into the broth. Additional tubes may be inoculated with 0.5 ml each from the suspension. Also, 0.1 ml may be inoculated onto a slant. Streak several blood plates to check for colonial morphology and purity.
4. Incubate tubes under an anaerobic atmosphere at 37oC. Incubate one agar plate anaerobically for colony formation, and one aerobically for aerobic contamination check.
5. Within 5 to 6 days, growth should be evident by turbidity in the broth. On agar surfaces, colonies are entire, glistening, smooth, high convex, circular, and translucent with a watery sheen. No growth should occur on the blood agar plate incubated aerobically.
Anaerobic conditions for transfer may be obtained by either of the following:
·Use of an anaerobic gas chamber, or
·Placement of test tubes under a gassing cannula system hooked to anaerobic gas.
· Loose screw caps on test tubes in anaerobic chamber,
· Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
· Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
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Haapasalo M, et al. Mitsuokella dentalis sp. nov. from dental root canals. Int. J. Syst. Bacteriol. 36: 566-568, 1986.
Willems A, Collins MD. 16S rRNA gene similarities indicate that Hallella seregens (Moore and Moore) and Mitsuokella dentalis (Haapsalo et al.) are genealogically highly related and are members of the genus Prevotella: emended description of the genus Prevotella (Shah and Collins) and description of Prevotella dentalis comb. nov.. Int. J. Syst. Evol. Microbiol. 45: 832-836, 1995. PubMed: 7547307