Desulfovibrio fructosivorans Ollivier et al.
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2. Under anaerobic conditions, withdraw 0.5 ml of #1249 broth from a single test tube (5 to 6 ml) and rehydrate the entire vial contents.
3. Aseptically transfer this aliquot back into the broth tube. A slant and a pre-reduced blood plate may also be inoculated with 0.1 ml each of the cell suspension. An aerobic blood plate may also be streaked to check for purity.
4. Incubate tubes and plate under anaerobic conditions at 37°C. Incubate blood plate aerobically at 37°C.
5. Within 5-7 days, growth should be evident by turbidity in the broth.
Anaerobic conditions for transfer may be obtained by either of the following:
· Use of an anaerobic gas chamber, or
· Placement of test tubes under a gassing cannula system hooked to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
· Loose screw caps on test tubes in anaerobic chamber,
· Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
· Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
(2.0 ml per 100 ml of medium).
Other commonly used reducing agents are sodium sulfide, cysteine, dithiothreitol, and titanium citrate. Cysteine is the reducing agent of choice since it does not cause the ferrous ammonium sulfate to precipitate.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
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Validation list no. 32. Int. J. Syst. Bacteriol. 40: 105-106, 1990.
Ollivier B, et al. Characterization of Desulfovibrio fructosovorans new species. Arch. Microbiol. 149: 447-450, 1988.