Streptococcus pneumoniae (Klein) Chester
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2. Using a single tube of #44 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #260 agar slant and/or plate.
5. Incubate the tubes and plate at 37oC for 24 hours in an atmosphere of 5% CO2. Screw caps should be loose.
This strain dies off readily and may require frequent transfers to fresh media to retain viability.
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