Eubacterium acidaminophilum Zindel et al.
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2. Under anaerobic conditions, withdraw 0.5 ml of recommended broth from a single test tube (5 to 6 ml) and rehydrate the vial contents.
3. Aseptically transfer this aliquot back into the broth tube. Mix well. A slant and an additional broth may be inoculated from this suspension. An aerobic blood plate may also be streaked to check for purity.
4. Incubate tubes under anaerobic conditions at 30°C. Incubate blood plate aerobically.
5. Within 5 days, growth should be evident by turbidity in the broth tube. No growth should occur on the blood agar plate incubated aerobically. Cells are large regular rods occurring singly, in pairs and in short chains.
Anaerobic conditions for transfer may be obtained by either of the following:
· Use of an anaerobic gas chamber, or
· Placement of test tubes under a gassing cannula system hooked to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
· Loose screw caps on test tubes in anaerobic chamber,
· Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
· Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
Always use freshly prepared pre-reduced media or pre-reduced media that has been previously prepared but stored under anaerobic conditions. Resazurin in the media is a color indicator for anaerobic conditions. Observance of pink color in medium before use or during incubation shows anaerobic conditions have not been met and oxidation has occurred. Medium should be discarded.
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Validation list no. 28. Int. J. Syst. Bacteriol. 39: 93-94, 1989.
Zindel U, et al. Eubacterium-acidaminophilum New-species a versatile amino acidi-degrading anaerobe producing or utilizing hydrogen or formate description and enzymatic studies. Arch. Microbiol. 150: 254-266, 1988.