Geobacillus thermoglucosidasius (Suzuki) Nazina et al.
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2. Using a single tube of #18 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate an additional broth tube, a #18 agar slant and/or a plate.
5. Incubate all tubes and plate at 55°C for 24 hours.
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Suzuki Y, et al. Bacillus thermoglucosidasius sp.nov., a new species of obligately thermophilic bacilli. Syst. Appl. Microbiol. 4: 487-495, 1983.
Validation list no. 14. Int. J. Syst. Bacteriol. 34: 270-271, 1984.
Nazina TN, et al. Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermoglucosidasius and Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. thermocatenulatus, G. thermoleovorans, G. kaustophilus, G. thermoglucosidasius and .... Int. J. Syst. Evol. Microbiol. 51: 433-446, 2001. PubMed: 11321089