Eubacterium yurii subsp. yurii Margaret and Krywolap
2. Under anaerobic conditions, withdraw 0.5 ml of recommended broth from a single test tube (5 to 6 ml) and rehydrate the vial contents.
3. Aseptically transfer this aliquot back into the broth tube. A slant and a pre-reduced blood plate may also be inoculated with 0.1 ml each of the cell suspension. An aerobic blood plate may also be streaked to check for purity.
4. Incubate tubes and one plate under anaerobic conditions at 37°C. Incubate one blood plate aerobically at 37°C.
5. Growth should appear in 24 to 48 hours.
Anaerobic conditions for transfer may be obtained by either of the following:
· Use of an anaerobic gas chamber, or
· Placement of test tubes under a gassing cannula system hooked to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
· Loose screw caps on test tubes in anaerobic chamber,
· Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
· Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
Always use freshly prepared pre-reduced media or pre-reduced media that has been previously prepared but stored under anaerobic conditions. Resazurin in the media is a color indicator for anaerobic conditions. Observance of pink color in medium before use or during incubation shows anaerobic conditions have not been met and oxidation has occurred. Medium should be discarded.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
Margaret BS, Krywolap GN. Eubacterium yurii subsp. yurii sp. nov. and Eubacterium yurii subsp. margaretiae subsp. nov.: Test tube brush bacteria from subgingival dental plaque. Int. J. Syst. Bacteriol. 36: 145-149, 1986.