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Clostridium difficile (Hall and O'Toole) Prevot

43593-FZ

Product category
Bacteria
Product type
Anaerobe
Strain designation
1351
Type strain
No
Isolation source
Feces
Geographical isolation
Belgium
Product format
Frozen
Storage conditions
-80°C or colder

Documentation

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Biosafety Icon BSL 2

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Preceptrol
No

Characteristics

Serotype
B
Toxigenic
No
Toxin genes
cdtB (Binary toxin) negative
tcdA (Toxin A) negative
tcdB (Toxin B) negative
Comments
Preparation of Clostridium difficile strain 1351 ATCC® 43593-FZ™, frozen for ease of use. Lot specific titers are available upon request.
Binary toxin gene cdtB not amplified by PCR
Toxinotype tcdA-, tcdB-
Ribotype 060

Handling information

Medium
Temperature
37°C
Atmosphere
Anaerobic
Handling procedure
  1. Thaw the frozen vial under anaerobic conditions.
  2. Under anaerobic conditions, transfer the entire contents of the thawed vial into a single tube (5-6 ml) of #2107 broth. Mix well.
  3. Additional tubes of broth may be inoculated with 0.5 ml each from the suspension and 0.1 ml may be inoculated onto a slant. Streak several blood plates for colony morphology and purity.
  4. Incubate the tubes and plate under anaerobic conditions at 37°C for 24 to 48 hours. Incubate the purity plate aerobically at 37°C. Turbid growth is evident in the broth at 24 to 48 hours.

 

ANAEROBIC CONDITIONS:

Anaerobic conditions for transfer may be obtained by either of the following:

  • Use of an anaerobic gas chamber, or
  • Placement of test tubes under a gassing cannula system connected to anaerobic gas.

 

Anaerobic conditions for incubation may be obtained by any of the following:

  • Loose screw caps on test tubes in anaerobic chamber,
  • Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
  • Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.

Handling notes
Anaerobe Systems Brucella Blood Agar Plates (AS-111) can be used to analyze colony morphology and purity.

Always use freshly prepared pre-reduced media or pre-reduced media that has been previously prepared but stored under anaerobic conditions.  Resazurin in the media is a color indicator for anaerobic conditions.  Observance of pink color in medium before use or during incubation shows anaerobic conditions has not been met and oxidation has occurred.  Medium should be discarded.

Additional information on this culture is available on the ATCC® web site at www.atcc.org.

History

Deposited as
Clostridium difficile (Hall and O'Toole) Prevot
Depositors
M Delmee
Type of isolate
Human

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

References

Curated Citations

Delmee M, et al. Serogrouping of Clostridium difficile strains by slide agglutination. J. Clin. Microbiol. 21: 323-327, 1985. PubMed: 3980688

Delmee M, et al. Comparison of serogrouping and polyacrylamide gel electrophoresis for typing Clostridium difficile. J. Clin. Microbiol. 24: 991-994, 1986. PubMed: 3782463

feces of asymptomatic neonate

Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303

Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986

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