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Methanocorpusculum labreanum Zhao et al.

43576

Type strain that has been genome sequenced
Product category
Bacteria
Product type
Extremophile
Strain designation
OCM 1 [DSM 4855, Z]
Type strain
Yes
Genome sequenced strain
Yes
Isolation source
Sediment
Geographical isolation
United States; California; Los Angeles
Product format
Frozen
Storage conditions
-80°C or colder
Mission Collection Item
This is a Mission Collection Item

Documentation

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Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Preceptrol
No

Characteristics

Extremophile type
Methanogen
Comments
Genome sequenced strain

Handling information

Medium
Temperature
37°C
Handling procedure
  1. Sterilize the top of the Balch tube by spraying it with 70% ethanol and then flame the top
  2. If needed exchange the gas in the test tube for 80% H2-20% CO2 or 80% N2-20% CO2.
  3. If the medium is pink (see discussion about resazurin) add 0.1 ml Co-enzyme M (5% stock solution, see below) per 10 ml of medium.  Let the medium sit at room temperature for a minimum of 30 minutes or up to 24 hours.  The resazurin must be colorless before inoculating.
  4. When the Balch tube is ready to inoculate, thaw the frozen vial at room temperature under a gentle stream of oxygen-free gas.
  5. For inoculation, use an anaerobic (see "c" below) 1.0 ml syringe tipped with 22-gauge needle, withdraw the cell suspension from the vial and transfer it to the broth.  Plate 0.1 ml of the inoculated culture onto a non-selective medium and incubate aerobically at 37°C.  Use 0.1 ml of the inoculated culture to inoculate a nonselective aerobic broth and an additional tube of #2487 broth.   Incubate the non-selective aerobic broth tubes at 37°C.  Incubate the anaerobic tube at 37°C.
  6. Growth should be detected in the #2487 broth within 48 to 72 hours.  There should be no growth detected on the aerobic plate or in the aerobic broth.

 

ANAEROBIC CONDITIONS:

  1. Resazurin is a commonly used redox indicator that is pink when the redox potential is above -50 mv., and colorless when the redox potential is below -110 mv. i.e. highly reducing.  Most strict anaerobes require this low redox potential for optimum growth.
  2. To obtain a fully reduced medium, it is necessary that the medium be anoxic and that a reducing agent be added.  Common reducing agents are sodium sulfide, cysteine, dithiothreitol, and titanium citrate.
  3. Syringes can be made anaerobic by one of two methods.  
    1. Displace the dead space in the syringe with a sterile oxygen-free gas.
    2. Displace the dead space in the syringe with a reducing agent. 
Handling notes

Additional information on this culture is available on the ATCC web site at www.atcc.org.

History

Deposited as
Methanogenium sp.
Depositors
DR Boone
Type of isolate
Environmental
Year of origin
1988
Special collection
NSF - Bacteriology
Cross references
GenBank CP000559 Methanocorpusculum labreanum Z, complete genome

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Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
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The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

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Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Zhao Y, et al. Isolation and characterization of Methanocorpusculum labreanum sp. nov. from the LaBrea Tar Pits. Int. J. Syst. Evol. Microbiol. 39: 10-13, 1989.

surface sediments collected from an active, gas-producing area of Tar Pit Lake, LaBrea Tar Pits, Los Angeles, CA

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