Thermonema lapsum Hudson et al.
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2. Using a single tube of #461 broth (5 to 6 ml), withdraw approximately 0.6 ml with a Pasteur or 1.0 ml pipette. Rehydrate the pellet.
3. Aseptically transfer 0.3 ml of this suspension back into the broth tube. Mix well.
4. Use the remaining 0.3 ml of the suspension to inoculate a slant of #461 agar.
5. Incubate tubes at 60oC. For best results, incubate tubes within a closed jar with a moistened paper towel added to maintain humidity and prevent desiccation.
6. Once growth is obtained in broth, plates can be inoculated with approximately 0.2 ml per plate. Plates should be incubated in a jar as described above or taped shut to prevent desiccation.
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Hudson JA, et al. Thermonema lapsum gen. nov., sp. nov., a thermophilic gliding bacterium. Int. J. Syst. Bacteriol. 39: 485-487, 1989.