Atopobium fossor (Bailey and Love) Kageyama et al.
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2. Under anaerobic conditions, withdraw 0.5 ml of recommended broth from a single test tube (5 to 6 ml) and rehydrate the entire vial contents.
3. Aseptically transfer this aliquot back into the broth tube. A slant and a #1490 or pre-reduced blood plate may also be inoculated with 0.1 ml each of the cell suspension. An aerobic blood plate may also be streaked to check for purity.
4. Incubate tubes and plate under anaerobic conditions at 37°C. Incubate blood plate aerobically at 37°C.
5. Growth should occur in broth in 24 to 48 hours. Growth may be slower on solid medium. Colonies on sheep blood agar are flat and glistening with irregular margins. No growth should appear on the aerobic plate.
Anaerobic conditions for transfer may be obtained by either of the following:
· Use of an anaerobic gas chamber, or
· Placement of test tubes under a gassing cannula system hooked to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
· Loose screw caps on test tubes in anaerobic chamber,
· Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
· Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
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Bailey GD, Love DN. Eubacterium fossor sp. nov., an agar-corroding organism from normal pharynx and oral and respiratory tract lesions of horses. Int. J. Syst. Bacteriol. 36: 383-387, 1986.