Heliobacterium gestii Ormerod et al.
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2. Fill a sterile small screw cap test tube close to capacity with ATCC medium 1552. Pre reduce the medium by adding 0.2 ml of a sterile 3 % Cysteine solution. Finish filling the test tube to capacity with ATCC medium 1552 and seal the test tube with the screw cap. Let the tube sit at room temperature for a minimum of 30 minutes, the test tube can sit over night without any adverse effects.
3. Rehydrate the entire pellet using 0.5 ml of the pre reduced medium from the small test tube.
4. Aseptically transfer this aliquot back into the broth tube. Make sure the test tube is filled to capacity.
5. Use several drops of the suspension to inoculate a nutrient or trypticase soy plate and streak out.
6. Incubate the tubes under a tungsten lamp at 37 oC. Incubate the plate(s) at 37 oC in the dark.
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Validation list no. 59. Int. J. Syst. Bacteriol. 46: 1189-1190, 1996.
Ormerod JG, et al. Heliophilum fasciatum gen. nov. sp. nov. and Heliobacterium gestii sp. nov.: endospore-forming heliobacteria from rice field soils. Arch. Microbiol. 165: 226-234, 1996.