Prevotella oulorum (Shah et al.) Shah and Collins
2. Under anaerobic conditions, withdraw 0.5 ml of the recommended broth from a single test tube (5 to 6 ml) and rehydrate the entire vial contents.
3. Aseptically transfer this aliquot back into the broth. Additional tubes may be inoculated with 0.5 ml each from the suspension. Also, 0.1 ml may be inoculated onto a slant. Streak several blood plates to check for colonial morphology and purity.
4. Incubate tubes under an anaerobic atmosphere at 37oC. Incubate one agar plate anaerobically for colony formation, and one aerobically for aerobic contamination check.
5. Within 24 to 48 hours, growth should be evident by turbidity in the broth. On agar surfaces, colonies are pinpoint, translucent, circular, entire, low convex, and they flatten with age. No growth should occur on the blood agar plate incubated aerobically.
Anaerobic conditions for transfer may be obtained by either of the following:
·Use of an anaerobic gas chamber, or
·Placement of test tubes under a gassing cannula system hooked to anaerobic gas.
· Loose screw caps on test tubes in anaerobic chamber,
· Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
· Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
Shah HN, et al. Bacteroides oulorum sp. nov., a nonpigmented saccharolytic species from the oral cavity. Int. J. Syst. Bacteriol. 35: 193-197, 1985.
Shah HN, Collins DM. Prevotella, a new genus to include Bacteroides melaninogenicus and related species formerly classified in the genus Bacteroides. Int. J. Syst. Bacteriol. 40: 205-208, 1990. PubMed: 2223612
. International Committee on Systematic Bacteriology Subcommittee on Gram-Negative Anaerobic Rods (Minutes of the Meeting, 13 and 14 September, 1990, Osaka, Japan). Int. J. Syst. Bacteriol. 41: 590-591, 1991.