Planctomyces limnophilus Hirsch and Muller
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2. Using a single tube of #1521 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Transfer 1.0 ml of the suspension to a second tube of broth. From the second tube, use several drops to inoculate a slant and/or plate if desired. The cryoprotectant used in the freeze‑drying procedure may inhibit growth in the primary tube, hence the necessity for immediate transfer.
5. Incubate all tubes and plate at 30oC for 7 to 10 days. If growth is not heavy on agar medium, make additional transfers at this time.
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Validation list no. 20. Int. J. Syst. Bacteriol. 36: 354-356, 1986.
Hirsch P, Muller M. Planctomyces limnophilus sp.nov., a stalked and budding bacterium from freshwater. Syst. Appl. Microbiol. 6: 276-280, 1985.