Thermus filiformis Hudson et al.
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2. Using a single tube of #461 broth (5 to 6 ml), withdraw approximately 0.6 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer 0.3 ml of this suspension back into the broth tube. Mix well.
4. Use the remaining 0.3 ml of the suspension to inoculate a slant of #461 agar.
5. Incubate tubes at 70oC. For best results, incubate tubes within a closed jar with a moistened paper towel added to maintain humidity and prevent desiccation.
6. Once growth is obtained in broth, plates can be inoculated with approximately 0.2 ml per plate. Better growth is obtained on plates when incubated at 60oC than at 70oC. Plates should be taped shut to prevent desiccation.
At 1000X magnification, cells are threadlike.
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Hudson JA, et al. Isolation and characterization of a new caldoactive filamentous bacterium. FEMS Microbiol. Lett. 22: 149-153, 1984.
Hudson JA, et al. Thermus filiformis sp. nov., a filamentous caloactive bacterium. Int. J. Syst. Bacteriol. 37: 431-436, 1987.