Desulfobulbus elongatus Samain et al.
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2. Perform all steps under anaerobic conditions.
3. Aseptically transfer 0.5 ml of #1555 broth to the vial and rehydrate the entire pellet. Transfer the suspension back into the broth tube. Inoculate a plate of a non-selective medium such as Trypticase Soy, Nutrient, or Blood agar with 0.1 ml of the cell suspension.
4. Seal the tube with a rubber stopper and incubate anaerobically at 37oC. Incubate the plate(s) aerobically as a contamination check.
5. Once growth is achieved, transfer the culture to fresh tubes of #1555 broth.
Anaerobic conditions for transfer may be obtained by either of the following:
· Use of an anaerobic gas chamber, or
· Placement of test tubes under a gassing cannula system connected to anaerobic gas or.
· Exchanging the headspace in stoppered culture tubes for the appropriate anaerobic gas
Anaerobic conditions for incubation may be obtained by any of the following:
· Loose screw‑caps on test tubes in anaerobic chamber,
· Loose screw‑caps on test tubes in an activated anaerobic Gas Pak jar, or
· Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
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Validation list no. 17. Int. J. Syst. Bacteriol. 35: 223-225, 1985.
Samain E, et al. Isolation and characterization of Desulfobulbus elongatus sp. nov. from a mesophilic industrial digester. Syst. Appl. Microbiol. 5: 391-401, 1984.