Psychrobacter immobilis Juni and Heym
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2. From a single tube of #44 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Use several drops of this suspension to inoculate an additional broth tube, a #44 agar slant and/or a plate.
5. Incubate the tubes and plate at 26°C for 24 hours.
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Juni E, Heym GA. Psychrobacter immobilis gen. nov., sp. nov.: Genospecies composed of gram-negative, aerobic, oxidase-positive Coccobacilli. Int. J. Syst. Bacteriol. 36: 388-391, 1986.
. . Appl. Environ. Microbiol. 40: 1106-1114, 1980.