Clostridium acetobutylicum McCoy et al. emend. Keis et al.
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2. Under anaerobic conditions, withdraw 0.5 ml of #1500 broth from a single test tube (5 to 6 ml) and rehydrate the entire vial contents.
3. Aseptically transfer this aliquot back into the broth tube. Additional tubes may be inoculated with 0.5 ml each from the suspension. Streak several blood plates to check for colonial morphology and purity.
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