Clostridium papyrosolvens Madden et al.
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2. Under anaerobic conditions, withdraw 0.5 ml of the
recommended broth from a single test tube (5 to 6 ml) and rehydrate the vial contents.
Additional tubes may be inoculated with 0.5 ml each from the suspension. 0.1 ml may also be inoculated onto a slant. Streak several blood plates to check for colonial morphology and purity.
Incubate one agar plate anaerobically for colony formation, and one aerobically for aerobic contamination check.
5. Within 7 days, growth should be evident by turbidity in
the broth and by irregular, convex, circular, slightly beta hemolytic colonies on the anaerobic agar surfaces. No growth should occur on agar plates incubated aerobically.
Anaerobic conditions for transfer may be obtained by either of the following:
·Use of an anaerobic gas chamber, or
·Placement of test tubes under a gassing cannula system hooked to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
·Loose screw caps on test tubes in anaerobic chamber,
·Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
·Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
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Madden RH, et al. Isolation and characterization of an anaerobic, cellulolytic bacterium, Clostridium papyrosolvens sp. nov.. Int. J. Syst. Bacteriol. 32: 87-91, 1982.