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Acidithiobacillus ferrooxidans (Temple and Colmer) Kelly and Wood

33020

Product category
Bacteria
Product type
Extremophile
Strain designation
11Fe
Type strain
No
Isolation source
Water from uranium mine
Geographical isolation
Japan
Product format
Test tube
Mission Collection Item
This is a Mission Collection Item.

Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Produces hydrogen sulfide:ferric ion oxidoreductase
Biotechnology
Preceptrol
No

Characteristics

Extremophile type
Acidophile
Metallotolerant
Sulfur oxidizer
Comments
Leaching of uranium from ore
Cloning of ntrBC genes

Handling information

Temperature
26°C
Handling procedure
1. Incubate test tube cultures under above conditions upon receipt.  It is helpful to incubate test tubes in a slanted position to increase gas exchange in broth.  Transfer culture to fresh media within one week of arrival.

2. Gently vortex the test-tube to dislodge the iron that has oxidized onto the glass.  Aseptically withdraw approximately 1.0 ml of the broth culture and transfer into 5 ml of fresh broth.

3. Incubate the broth either on a shaker or in a static and slanted position.

4. Growth is evident within one week, when yellow-orange iron oxide deposits are observed.

5. Transfer the culture approximately every two weeks.

1. Incubate test tube cultures under above conditions upon receipt.  It is helpful to incubate test tubes in a slanted position to increase gas exchange in broth.  Transfer culture to fresh media within one week of arrival.

2. Gently vortex the test-tube to dislodge the iron that has oxidized onto the glass.  Aseptically withdraw approximately 1.0 ml of the broth culture and transfer into 5 ml of fresh broth.

3. Incubate the broth either on a shaker or in a static and slanted position.

4. Growth is evident within one week, when yellow-orange iron oxide deposits are observed.

5. Transfer the culture approximately every two weeks.

Handling notes
The cells are motile and rod-shaped.

          Additional information on this culture is available on the ATCC web site at www.atcc.org.

The cells are motile and rod-shaped.

          Additional information on this culture is available on the ATCC web site at www.atcc.org.

History

Deposited as
Thiobacillus ferrooxidans Temple and Colmer
Depositors
HM Tsuchiya
Chain of custody
ATCC <-- HM Tsuchiya <-- N. Tomizuka
Type of isolate
Environmental
Cross references
GenBank X95571 T.ferrooxidans alaS gene.
GenBank M26933 T.ferrooxidans recA gene, complete cds.
GenBank M16626 T.ferrooxidans glnA gene encoding glutamine synthetase, complete cds.
GenBank M58480 Thiobacillus ferrooxidans nitrogen metabolism regulator (ntrA) gene, complete cds.
GenBank AJ006456 Thiobacillus ferrooxidans cyc1, cyc2, cox1, cox2, cox3, cox4 and rus genes and open reading frame.
GenBank L18975 Thiobacillus ferrooxidans histidine phosphokinase/phophatase (ntrB) and DNA-binding protein (ntrC) genes, complete cds's.
GenBank U20361 Thiobacillus ferrooxidans thioredoxin (trxA) gene, complete cds and rho transcription terminator (rho) gene, partial cds.
GenBank M15238 T.ferrooxidans nifHDK operon encoding nitrogenase reductase and molybdenum-iron protein alpha and beta subunits, complete cds.
GenBank AF032884 Thiobacillus ferrooxidans N-acetylglucosamine-1-phosphate uridyltransferase (glmU) gene, partial cds; glucosamine synthase (glmS) and RecG (recG) genes, complete cds; and transposon Tn5468, complete sequence.
GenBank M81087 Thiobacillus ferrooxidans F1F0-ATPase c subunit (atpE) gene, b subunit (atpF) gene, delta subunit (atpH) gene, alpha subunit (atpA) gene, gamma subunit (atpG) gene, beta subunit (atpD) gene, epsilon subunit (atpC) gene, complete cds's.
GenBank U81808 Thiobacillus ferrooxidans pyruvate dehydrogenase complex E1 alpha subunit (pdhA), pyruvate dehydrogenase complex E1 beta subunit (pdhB), pyruvate dehydrogenase complex dihydrolipoyl transacetylase/lipoamide dehydrogenase (pdhC), putative citrate

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Sugio T, et al. Existence of a hydrogen sulfide:Ferric ion oxidoreductase in iron-oxidizing bacteria. Appl. Environ. Microbiol. 58: 431-433, 1992.

. . Proc. IV IFS: Ferment. Technol. Today : 513-520, 1972.

Agric. Biol. Chem. 40: 1019-1025, 1976.

Kilkenny CA, et al. Isolation of the Thiobacillus ferrooxidans ntrBC genes using a T. ferrooxidans nifH-lacZ fusion. Microbiology 140: 2543-2553, 1994. PubMed: 8000526

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