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Thermoanaerobacter ethanolicus Wiegel and Ljungdahl


Thermoanaerobacter ethanolicus strain JW 200 was isolated in 1978 from a hot spring at Yellowstone National Park. This thermophilic type strain is propagated anaerobically on Thermoanaerobacter ethanolicus medium at 55°C. 
Product category
Product type
Strain designation
JW 200 [DSM 2246]
Type strain
Isolation source
Hot spring at Yellowstone National Park
Geographical isolation
United States; Wyoming
Product format
Storage conditions
2°C to 8°C
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
Degrades cellulose
Produces alcohol dehydrogenase
Produces ethyl alcohol ethanol
Production of ethanol from cellulose


Extremophile type
Technical information
ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.

Handling information

Handling procedure
  1. Open vial according to enclosed instructions. 
  2. Perform all steps under anaerobic conditions (see below).
  3. Aseptically transfer 0.5 ml of ATCC Medium #1190 to the vial and rehydrate the freeze-dried pellet, immediately place the rehydrated pellet under a stream of oxygen-free sterile gas.  Then transfer the suspension back into the tube of broth.  Inoculate a plate of non-selective medium with 0.1 of the culture.  Inoculate a non-selective tube of broth.
  4. Seal the test tube with a rubber stopper and incubate anaerobically at 55°C.  The rubber stoppers should be tapped down to insure that the anaerobic conditions are maintained during incubation.  Incubate the plate(s) and aerobic broth at 37°C as a purity check.
  5. After one or three days, growth should be evident by turbidity through out the broth.  Once growth has been established, the culture should be transferred to fresh broth every 24 to 48 hours.
  6. This culture is very sensitive to oxygen; therefore steps should be taken to avoid exposure to oxygen.  When the culture exhibits good growth it will remain viable for up to 1 week if stored at 4°C under anaerobic conditions.



  • Tubes of media are placed under a gassing cannula system hooked to a source of oxygen free gas.
  • All transfers are performed while the test tubes are on the cannula system with a gentle stream of oxygen-free gas flowing through the system.
  • As the test tubes are removed from the cannula system each is sealed with butyl rubber stopper thus maintaining the anaerobic headspace.


Deposited as
Thermoanaerobacter ethanolicus Wiegel and Ljungdahl
LG Ljungdahl
Patent depository
This material was deposited with the ATCC Patent Depository to fulfill U.S. or international patent requirements. This material may not have been produced or characterized by ATCC.  As an International Depository Authority (IDA) for patent deposits, ATCC is required to complete viability testing only at time of initial deposit of patent material. Patent deposits are made available on behalf of the Depositor when the pertinent U.S. or international patent is issued, but material may not be used to infringe the patent claims.
Patent number
Type of isolate
Year of origin
Special collection
National Park Service (NPS) Special Collection
Cross references
GenBank L09162 Thermoanaerobacter ethanolicus (ATCC 31550) 16S ribosomal RNA (16S rRNA) gene.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

This material is cited in a US and/or international patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Depositor of the party to which the material was furnished.

Permits & Restrictions

Material Transfer Agreement Addendum for National Park Service Products

For every order of this item, you must provide a signed Material Transfer Agreement Addendum for National Park Service Products. We cannot ship this item until we receive this addendum. The person signing the addendum as the principal investigator must match the end user as listed on the applicable sales order for the item. Email the signed addendum to [email protected] with a reference to both your account and sales order numbers. Once received, your addendum will be reviewed, and this item will be released for shipment if all requirements are met. If you need assistance with your order, please contact our Customer Care team or your applicable distributor.

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.



Curated Citations

Bryant FO, et al. Purification and properties of primary and secondary alcohol dehydrogenases from Thermoanaerobacter ethanolicus. Appl. Environ. Microbiol. 54: 460-465, 1988.

Ljungdahl LG, Wiegel JK. Anaerobic thermophilic culture system. US Patent 4,292,406 dated Sep 29 1981

Wiegel J, Ljungdahl LG. Thermoanaerobacter ethanolicus, new genus new species a new, extreme thermophilic, anaerobic bacterium. Arch. Microbiol. 128: 343-348, 1981.

Validation list no. 9. Int. J. Syst. Bacteriol. 32: 384-385, 1982.

type strain

View All Curated Citations for this Product

Frequently Asked Questions

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