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Tritrichomonas foetus (Riedmuller) Wenrich and Emerson

30924

Product category
Protists
Product type
Parasitic protozoan
Drug-resistant protozoan
Strain designation
KV-1
Type strain
No
Isolation source
Naturally infected Bos taurus
Geographical isolation
Czech Republic; Zalmanov
Applications
Agricultural research
Antimicrobial resistance research
Drug development
Product format
Frozen
Storage conditions
-80°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
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Biosafety Icon BSL 2

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information

General

Specific applications
produces glucokinase
produces ketohexokinase fructokinase

Characteristics

Susceptibility profile
Resistant
Metronidazole
Comments
Phylogeny based upon superoxide dismutase gene sequence analysis
Glucokinase and fructokinase
Fatty acid and sterol metabolism
phospholipid metabolism
phylogeny
Behavior and pathogenicity
Tubulin subunits and phylogeny
Metronidazole resistant activities of hydrogenosomal enzymes
Subcellular localization of enzymes of the arginine dihydrolase pathway
Fructose-2,6-bisphosphate-insensitive pyrophosphate:fructose-6-phosphate phosphotransferase

Handling information

Medium
Instruction for complete medium
Media: ATCC Medium 2154 (ATCC Medium 2154 is available in a freeze-dried format as cat. no. PRA-2154).

Alternate Media: ATCC Medium 359
Temperature
35°C
Culture system
Axenic
Handling procedure
Storage and Culture Initiation
Frozen ampules packed in dry ice should either be thawed immediately or stored in liquid nitrogen.  If liquid nitrogen storage facilities are not available, frozen ampules may be stored at or below -70°C for approximately one week.  Do not under any circumstance store frozen ampules at refrigerator freezer temperatures (generally -20°C).  Storage of frozen material at this temperature will result in the death of the culture.
  1. To thaw a frozen ampule, place it in a 35°C water bath , until thawed (2-3 min).  Immerse the ampule just sufficient to cover the frozen material.  Do not agitate the ampule.
  2. Immediately after thawing, aseptically transfer contents to a screw-capped test tube containing either 9 mL of ATCC medium 359 (completed with serum) or 13 mL ATCC Medium 2154.  Incubate the tube at 35°C (tube should be vertical for medium 359 or on a 15° horizontal slant for medium 2154).
Culture maintenance
  1. When the culture is at or near peak density, place the tubes on ice for 10 minutes. 
  2. Gently invert the culture tube 10 times and aseptically transfer a 0.1-0.4 mL aliquot to a screw-capped test tube containing either 9 mL of ATCC medium 359 (completed with serum) or 13 mL ATCC Medium 2154. 
  3. Incubate the culture at 35°C (tube should be vertical for medium 359 or on a 15° horizontal slant for medium 2154). 
  4. Transfer the culture every 3-4 days as described in steps 1-2.  The transfer interval will depend on the quantity of the inoculum and the quality of the medium.  This should be empirically determined by examining the culture on a daily basis until the growth cycle has stabilized. Do not allow the culture to overgrow. The culture crashes soon after reaching peak density.
Cryopreservation
  1. Harvest cells from a culture that is at or near peak density by centrifugation at 800 x g for 5 min. When cells grown in a medium containing agar are concentrated by centrifugation, a solid pellet does not form. The soft pellet is resuspended to desired cell concentration with agar-free supernatant.
  2. Adjust the concentration of cells to 2 x 106 - 2 x 107/mL in fresh medium.
  3. While cells are centrifuging prepare a 10% (v/v) solution of sterile DMSO in fresh medium.
    1. Add 1.0 mL of DMSO to an ice cold 20 x 150 mm screw-capped test tube;
    2. Place the tube on ice and allow the DMSO to solidify (~5 min) and then add 9.0 mL of ice cold medium;
    3. Invert several times to dissolve the DMSO;
    4. Allow to warm to room temperature.
  1. Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be 106 - 107 cells/mL and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should no less than 15 min and no longer than 30 min.
  2. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  3. Place the vials in a controlled rate freezing unit.  From room temperature cool at -1°C/min to -40°C.  If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion.  At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.  Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately -1°C/min.)  
  4. The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials should not be stored above -55°C.
  5. To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just above the surface of the frozen material. Do not agitate the vial.
  6. Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate a 16 x 125 mm screw-capped test tube containing either 9 mL of ATCC medium 359 (completed with serum) or 13 mL ATCC Medium 2154.
  7. Incubate the culture at 35°C with the cap screwed on tightly (tube should be vertical for medium 359 or on a 15° horizontal slant for medium 2154).

History

Deposited as
Tritrichomonas foetus (Riedmuller) Wenrich and Emerson
Depositors
BM Honigberg, J Kulda
Chain of custody
ATCC <-- BM Honigberg, J Kulda <-- E. Lipova
Type of isolate
Animal
Year of origin
1962
Cross references
GenBank Z70668 T.foetus sod1 gene.
GenBank Z70669 T.foetus sod2 gene.
GenBank AJ249457 mRNA for centrin (ce1 gene)
GenBank U17509 Tritrichomonas foetus 16S-like rRNA gene
GenBank AF307994 Tritrichomonas foetus cytosolic malate dehydrogenase 1 (MDH1) mRNA, partial cds
GenBank AF307995 Tritrichomonas foetus cytosolic malate dehydrogenase 2 (MDH2) mRNA, partial cds
GenBank AF022415 Tritrichomonas foetus glyceraldehyde-3-phosphate dehydrogenase (gap1) gene, partial cds.
GenBank AF022416 Tritrichomonas foetus glyceraldehyde-3-phosphate dehydrogenase (gap2) gene, partial cds.
GenBank AF312935 Tritrichomonas foetus ferredoxin mRNA, partial cds; nuclear gene for hydrogenosomal protein
GenBank AF312932 Tritrichomonas foetus hydrogenase mRNA, complete cds; nuclear gene for hydrogenosomal protein
GenBank AF312933 Tritrichomonas foetus malic enzyme mRNA, partial cds; nuclear gene for hydrogenosomal protein
GenBank AF312931 Tritrichomonas foetus succinyl CoA synthetase beta subunit; nuclear gene for hydrogenosomal protein
GenBank AF312930 Tritrichomonas foetus ATP/ADP carrier mRNA, partial sequence; nuclear gene for hydrogenosomal protein
GenBank L08622 Tritrichomonas foetus hypoxanthine-guanine-xanthine phosphoribosyltransferase (hpt) gene, complete cds.
GenBank AF312934 Tritrichomonas foetus pyruvate:ferredoxin oxidoreductase, partial sequence; nuclear gene for hydrogenosomal
GenBank AF312929 Tritrichomonas foetus succinyl CoA synthetase alpha subunit mRNA, partial cds; nuclear gene for hydrogenosomal protein

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

United States Veterinary Permit for Importation and Transportation of Controlled Materials and Organisms and Vectors

For every order of this item, you must provide a valid Permit for Importation and Transportation of Controlled Materials and Organisms and Vectors (VS Form 16-6A) obtained from the United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service. We cannot ship this item until we receive this permit.

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Beach DH, et al. Phospholipid metabolism of cultured Trichomonas vaginalis and Tritrichomonas foetus. Mol. Biochem. Parasitol. 44: 97-108, 1991. PubMed: 2011157

Beach DH, et al. Fatty acid and sterol metabolism of cultured Trichomonas vaginalis and Tritrichomonas foetus. Mol. Biochem. Parasitol. 38: 175-190, 1990. PubMed: 2325705

Kulda J, Honigberg BM. Behavior and pathogenicity of Tritrichomonas foetus in chick liver cell cultures. J. Protozool. 16: 479-495, 1969. PubMed: 5343462

Mertens E, Muller M. Glucokinase and fructokinase of Trichomonas vaginalis and Tritrichomonas foetus. J. Protozool. 37: 384-388, 1990. PubMed: 2213652

Gunderson J, et al. Phylogeny of trichomonads inferred from small-subunit rRNA sequences. J. Eukaryot. Microbiol. 42: 411-415, 1995. PubMed: 7620466

View All Curated Citations for this Product

Frequently Asked Questions

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