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Quantitative Genomic DNA from Trichomonas vaginalis strain C-1:NIH

30001DQ

Quantitative genomic DNA from Trichomonas vaginalis strain C-1:NIH can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine protozoan load.
Product category
Protists
Product type
Nucleic acid
Molecular standard
Derived from
Trichomonas vaginalis C-1:NIH (ATCC 30001)
Organism
Trichomonas vaginalis Donne
Type strain
No
Applications
Assay development
Infectious disease research
Next-generation sequencing
Sexually transmitted disease research
Volume
100 μL
Product format
Frozen
Storage conditions
-20°C or colder
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information

General

Specific applications
ATCC® Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine protozoan load.

Characteristics

Comments

Genomic DNA isolated from a preparation of Trichomonas vaginalis strain C-1:NIH (ATCC® 30001™). The source organism is also available through the ATCC catalog.

DNA quantitated by PCR.

Handling information

Handling procedure
  1. Thaw the vial at room temperature and immediately place on ice. Avoid exposing the DNA to repeated freeze-thaw cycles as it may result in degradation.
  2. Gently mix the sample to ensure an even distribution of material.
  3. Briefly centrifuge the tube before opening to ensure all liquid is at the bottom.
Handling notes
Aliquoting is highly recommended to avoid multiple freeze-thaws.

History

Depositors
ATCC
Cross references
GenBank Z70670 T.vaginalis sod1 gene.
GenBank Z70671 T.vaginalis sod2 gene.
GenBank Z70672 T.vaginalis sod3 gene.
GenBank Z70673 T.vaginalis sod4 gene.
GenBank Z70674 T.vaginalis sod5 gene.
GenBank M33717 T.vaginalis ferredoxin gene, complete cds.
GenBank U57000 chaperonin 60 (cpn60) mRNA, partial coding sequence
GenBank AF067404 DNA polymerase epsilon gene, partial coding sequence
GenBank X98016 histone H4-2 and histone H3-2, partial coding sequence
GenBank U27577 Trichomonas vaginalis polyubiquitin (UbA) mRNA, partial cds.
GenBank U28008 Trichomonas vaginalis ubiquitin 1A (Ub1A) gene, partial cds.
GenBank U28009 Trichomonas vaginalis ubiquitin 1C (Ub1C) gene, partial cds.
GenBank U28010 Trichomonas vaginalis ubiquitin 1D (Ub1D) gene, partial cds.
GenBank U28011 Trichomonas vaginalis ubiquitin 1E (Ub1E) gene, partial cds.
GenBank U28012 Trichomonas vaginalis ubiquitin dimer 2B (Ub2B) gene, partial cds.
GenBank U70308 Trichomonas vaginalis mitochondrial-type HSP70 mRNA, complete cds.
GenBank U38692 Trichomonas vaginalis cytosolic malate dehydrogenase gene, complete cds.
GenBank U28013 Trichomonas vaginalis polyubiquitin junction JC (UbJC) gene, partial cds.
GenBank AF060233 Trichomonas vaginalis L-lactate dehydrogenase (LDH1) gene, complete cds.
GenBank AF058282 Trichomonas vaginalis elongation factor 1 alpha (tef1) mRNA, partial cds.
GenBank L11394 Trichomonas vaginalis glyceraldehyde 3-phosphate dehydrogenase mRNA, 3' end.
GenBank U07784 Trichomonas vaginalis ATCC 30001 ferredoxin (FD) gene, Inr promoter element.
GenBank M97553 Trichomonas vaginalis succinyl-CoA synthetase beta-subunit gene, complete cds.
GenBank U07785 Trichomonas vaginalis ATCC 30001 P-glycoprotein (Pgp1) gene, promoter element.
GenBank AF022421 glyceraldehyde-3-phosphate dehydrogenase (gap3) gene, partial coding sequence
GenBank U07782 Trichomonas vaginalis ATCC 30001 beta-tubulin (beta-Tub) gene, promoter element.
GenBank U07780 Trichomonas vaginalis ATCC 30001 alpha-tubulin (alpha-Tub) gene, promoter element.
GenBank U07203 Trichomonas vaginalis hydrogenosomal adenylate kinase proprotein gene, complete cds.
GenBank U16836 Trichomonas vaginalis hydrogenosomal malic enzyme subunit A (maeA) gene, complete cds.
GenBank AF022414 Trichomonas vaginalis glyceraldehyde-3-phosphate dehydrogenase (gap2) gene, partial cds.
GenBank AF005075 translation initiation factor 2 gamma subunit (eIF-2 gamma) gene, partial coding sequence
GenBank U38786 calmodulin (CAM) and E2 ubiquitin-conjugating enzyme (TvUBC) genes, partial coding sequence
GenBank AF053370 PPi-dependent fructose 6-phosphate 1-phosphotransferase (pfk3) gene, partial coding sequence
GenBank U16822 Trichomonas vaginalis pyruvate:ferredoxin oxidoreductase proprotein (pfoA) gene, complete cds.
GenBank U16823 Trichomonas vaginalis pyruvate:ferredoxin oxidoreductase proprotein (pfoB) gene, complete cds.
GenBank U16838 Trichomonas vaginalis hydrogenosomal malic enzyme subunit C proprotein (maeC) gene, partial cds.
GenBank U16839 Trichomonas vaginalis hydrogenosomal malic enzyme subunit D proprotein (maeD) gene, partial cds.
GenBank U16837 Trichomonas vaginalis hydrogenosomal malic enzyme subunit B proprotein (maeB) gene, complete cds.
GenBank U07783 Trichomonas vaginalis ATCC 30001 70kDa cystolic heat shock protein (cHSP70 gene), promoter element.
GenBank AF044973 pyrophosphate-dependent fructose 6-phosphate 1-phosphotransferase (Pfk1) gene, complete coding sequence
GenBank AF053371 Trichomonas vaginalis PPi-dependent fructose 6-phosphate 1-phosphotransferase (pfk2) gene, partial cds.
GenBank U07781 Trichomonas vaginalis ATCC 30001 succinyl CoA synthetase beta subunit (beta-SCS) gene, Inr promoter element.
GenBank U07779 Trichomonas vaginalis ATCC 30001 succinyl CoA synthetase alpha subunit (alpha-SCSB) gene, Inr promoter element.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Searle SM, Muller M. Inorganic pyrophosphatase of Trichomonas vaginalis. Mol. Biochem. Parasitol. 44: 91-96, 1991. PubMed: 1849232

Lloyd D, Pedersen JZ. Metronidazole radical anion generation in vivo in Trichomonas vaginalis: oxygen quenching is enhanced in a drug-resistant strain. J. Gen. Microbiol. 131: 87-92, 1985. PubMed: 2985740

Jenkins TM, et al. Hydrogenosomal succinate thiokinase in Tritrichomonas foetus and Trichomonas vaginalis. Biochem. Biophys. Res. Commun. 179: 892-896, 1991. PubMed: 1898409

Mertens E, Muller M. Glucokinase and fructokinase of Trichomonas vaginalis and Tritrichomonas foetus. J. Protozool. 37: 384-388, 1990. PubMed: 2213652

Reardon LV, et al. Differences in strains of Trichomonas vaginalis as revealed by intraperitoneal injections into mice. J. Parasitol. 47: 527-532, 1961. PubMed: 13740097

View All Curated Citations for this Product

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