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Ruminococcus callidus Holdeman and Moore


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Ruminococcus callidus strain VPI S7-31 is a bacterial type strain that was isolated from human feces. This strain is propagated anaerobically and grows best when meat particles are left in the broth and sterile rumen fluid is added at the time of inoculation
Product category
Product type
Strain designation
VPI S7-31
Type strain
Genome sequenced strain
Isolation source
Product format
Storage conditions
-80°C or colder
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information



Handling information

Handling procedure
  1. Keep vial frozen until ready to use. 
  2. For optimal growth, do not filter out the meat particles when preparing the #1016 broth.
  3. To reduce media before inoculation, use 3.0% Cysteine then gas out vessel. 
  4. Add 10% by volume of sterile rumen fluid (0.5 mL per 5 mL broth) before inoculation. 
  5. Under anaerobic conditions, preferably in an anaerobe chamber, thaw vial and then quickly transfer entire contents into a single tube of #1016 broth. A second tube of #1016 broth can also be inoculated with 0.2 mL from the original broth.
  6. Incubate at 37°C. Initial growth may be observed at 48 hours. Subsequent transfers in broth, may grow faster. 
  7. Add 0.1 to 0.2 mL sterile rumen fluid to each Brucella blood plate prior to inoculation. Growth on agar may take up to 72 hours. It may be necessary to inoculate plates from a growing broth culture.


  1. Resazurin is a commonly used redox indicator that is pink when the redox potential is above -50 mv, and colorless when the redox potential is below -110 mv, ie, highly reducing. Most strict anaerobes require this low redox potential for optimum growth.
  2. To obtain a fully reduced medium, it is necessary that the medium be anoxic and that a reducing agent be added. There are several common reducing agents, however, 3.0% cysteine is recommended for this strain. Add 0.1 to 0.2 mL of reducing agent for each tube of medium. 
  3. Syringes can be made anaerobic by one of two methods.
    1. Displace the dead space in the syringe with a sterile oxygen-free gas.
    2. Displace the dead space in the syringe with a reducing agent.
Handling notes
Optimal growth is achieved by leaving meat particles in the broth and adding sterile rumen fluid at the time of inoculation. 
Anaerobe Systems Brucella Blood Plates (AS-111 or AS-141) are recommended for growth and colony morphology. 
Growth may have to be established in broth prior to transferring to agar.
Additional information on this culture is available on the ATCC® web site at

Quality control specifications

Verification method
Whole-genome Sequencing


Deposited as
Ruminococcus callidus Holdeman and Moore
LV Holdeman
Type of isolate
Cross references
GenBank L76596 Ruminococcus callidus small subunit ribosomal RNA (16S rDNA) gene.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

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