Trichoderma reesei Simmons
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Potential biofuel production agent: lignocellulosic ethanol
2. From a single test tube of sterile distilled water (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
3. Aseptically transfer the suspension back into the test tube of sterile distilled water.
4. Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; overnight rehydration is recommended.
5. Mix the suspension well. Use several drops to inoculate recommended solid or liquid medium.
6. Incubate cultures at recommended temperature.
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Messner R, et al. Differential regulation of synthesis of multiple forms of specific endoglucanases by Trichoderma reesei QM9414. J. Bacteriol. 170: 3689-3693, 1988. PubMed: 3403510
Mandels M, et al. Enhanced cellulase production by a mutant of Trichoderma viride. Appl. Microbiol. 21: 152-154, 1971. PubMed: 5099761