Bifidobacterium pseudolongum subsp. globosum (Scardovi et al.) Yaeshima et al.
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2. Under anaerobic conditions, withdraw 0.5 ml of #1053 from a single test tube (5 to 6 ml) and rehydrate the vial contents.
3. Aseptically transfer this aliquot back into the broth tube. Additional tubes may be inoculated with 0.5 ml each from the suspension. A slant of #1053 may also be inoculated with 0.2 ml. Streak several blood plates to check for colonial morphology and purity.
4. Incubate tubes under an anaerobic atmosphere at 37oC. Incubate one agar plate anaerobically for colony formation, and one aerobically for aerobic contamination check.
5. After 3 days, growth should be evident by tiny translucent colonies on the agar surfaces. The aerobic plate may have a thin film in the area of heaviest inoculation, however, it should show no other signs of aerobic contamination.
Anaerobic conditions for transfer may be obtained by either of the following:
· Use of an anaerobic gas chamber, or
· Placement of test tubes under a gassing cannula system hooked to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
· Loose screw caps on test tubes in anaerobic chamber,
· Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
· Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
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Scardovi V, et al. Deoxyribonucleic acid homology relationships among species of the genus Bifidobacterium. Int. J. Syst. Bacteriol. 21: 276-294, 1971.
Biavati B, et al. Electrophoretic patterns of proteins in the genus Bifidobacterium and proposal of four new species. Int. J. Syst. Bacteriol. 32: 358-373, 1982.
Validation list no. 43. Int. J. Syst. Bacteriol. 42: 656-657, 1992.
Scardovi V, et al. Bifid bacteria in bovine rumen. New species of the genus Bifidobacterium: B. globosum n. sp. and B. ruminale n. sp.. Arch. Mikrobiol. 68: 278-294, 1969. PubMed: 5393670
Yaeshima T, et al. Bifidobacterium globosum, subjective synonym of Bifidobacterium pseudolongum, and description of Bifidobacterium pseudolongum subsp. pseudolongum comb. nov. and Bifidobacterium pseudolongum subsp. globosum comb. nov.. Syst. Appl. Microbiol. 15: 380-385, 1992.