Clostridium indolis McClung and McCoy
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2. Under anaerobic conditions, withdraw 0.5 ml of #1053 broth from a single test tube (5 to 6 ml) and rehydrate the entire vial.
3. Aseptically transfer this aliquot back into the broth tube. Additional tubes may be inoculated with 0.5 ml each from the suspension. A slant of #1053 may also be inoculated with 0.2 ml. Streak several blood plates to check for colonial morphology and purity.
4. Incubate tubes under an anaerobic atmosphere at 37oC. Incubate one agar plate anaerobically for colony formation, and one aerobically for aerobic contamination check.
Anaerobic conditions for transfer may be obtained by
either of the following:
· Use of an anaerobic gas chamber, or
· Placement of test tubes under a gassing cannula system
hooked to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
· Loose screw caps on test tubes in anaerobic chamber,
· Loose screw caps on test tubes in an activated anaerobic
gas pack jar, or
· Use of sterile butyl rubber stoppers on test tubes so that
an anaerobic gas headspace is retained.
Colonies on the anaerobic blood agar plate are < 1mm, smooth, opaque, butyrous, circular, convex, entire, and white.
The cells are Gram variable rods, medium to large-size, of medium length, straight, in singles and pairs. The vegetative cells decolorize easily and usually are seen as being Gram negative. Also the spores are very difficult to detect and may not be observed. Cells are motile.
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Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.