Eubacterium contortum (Prevot) Holdeman et al.
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2. Under anaerobic conditions, withdraw 0.5 ml of recommended broth from a single test tube (5 to 6 ml) and rehydrate the entire vial contents.
3. Aseptically transfer this aliquot back into the broth tube. A slant and additional broth tubes may be inoculated with 0.2 ml each of the cell suspension. Blood plates may be streaked to check for colonial morphology and purity.
4. Incubate tubes and one plate under anaerobic conditions
at 37°C. Incubate one blood plate in 5% CO2 or in air at 37°C.
5. Within 48 hours, growth should be evident by turbidity in the broth and growth on agar surfaces. On #260 agar incubated anaerobically, colonies are entire, glistening, circular, smooth, and low convex. No growth should occur on the blood agar plate incubated aerobically.
Anaerobic conditions for transfer may be obtained by either of the following:
· Use of an anaerobic gas chamber, or
· Placement of test tubes under a gassing cannula system hooked to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
· Loose screw caps on test tubes in anaerobic chamber,
· Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
· Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
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Holdeman LV, et al. Eubacterium contortum (Prevot) comb. nov.: Emendation of description and designation of the type strain. Int. J. Syst. Bacteriol. 21: 304-306, 1971.
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.