Paracoccus versutus (Harrison) Katayama et al.
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2. Using a single tube of #472 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #472 agar slant and/or plate.
5. Incubate the tubes and plate at 26°C for 48-72 hours.
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Claassen PA, et al. Tricarboxylic-acid and glyoxylate cycle enzyme activities in Thiobacillus versutus an isocitrate lyase negative organism. Arch. Microbiol. 145: 148-152, 1986.
Harrison AP Jr.. Genomic and physiological comparisons between heterotrophic thiobacilli and Acidiphilum cryptum, Thiobacillus versutus sp. nov., and Thiobacillus acidophilus nom. rev.. Int. J. Syst. Bacteriol. 33: 211-217, 1983.
Taylor BF, Hoare DS. New facultative Thiobacillus and a reevaluation of the heterotrophic potential of Thiobacillus novellus. J. Bacteriol. 100: 487-497, 1969. PubMed: 5344108
Lu WP, Kelly DP. Purification and some properties of two principal enzymes of the thiosulphate-oxidizing multi-enzyme system from Thiobacillus A2. J. Gen. Microbiol. 129: 3549-3565, 1983.
Katayama Y, et al. Paracoccus thiocyanatus sp. nov., a new species of thiocyanate-utilizing facultative chemolithotroph, and transfer of Thiobacillus versutus to the genus Paracoccus as Paracoccus versutus comb. nov. with emendation of the genus. Microbiology 141: 1469-1477, 1995. PubMed: 7545513