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Paracoccus versutus (Harrison) Katayama et al.

25364

Product category
Bacteria
Product type
Extremophile
Strain designation
A2 [CCM 2505, DSM 582, IAM 12814, IFO 14567]
Type strain
Yes
Isolation source
Soil
Geographical isolation
United States; Texas
Product format
Freeze-dried
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Preceptrol
No

Characteristics

Comments
Enzyme patterns

Handling information

Medium
Temperature
26°C
Handling procedure
1. Open vial according to enclosed instructions. 

2. Using a single tube of #472 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette.  Rehydrate the entire pellet.

3. Aseptically transfer this aliquot back into the broth tube.  Mix well.

4. Use several drops of the suspension to inoculate a #472 agar slant and/or plate.

5. Incubate the tubes and plate at 26°C for 48-72 hours.

Handling notes
After 48 hours, colonies on #472 plates are circular, rough, flat, and transparent.

          Additional information on this culture is available on the ATCC® web site at www.atcc.org.

History

Deposited as
Thiobacillus versutus Harrison
Depositors
DS Hoare
Type of isolate
Environmental
Cross references
GenBank D32243 16S rRNA sequence
GenBank M11543 Thiobacillus versutus 5S ribosomal RNA.
GenBank Y16932 Paracoccus versutus 16S rRNA gene, strain ATCC 25364.
GenBank AY014174 Paracoccus versutus 16S ribosomal RNA gene, partial sequence.
GenBank X62808 T.versutus genes cyc and coxI for cytochrome c(550) and cytochrome-c oxidase subunit I.
GenBank L08575 Thiobacillus versutus methylamine dehydrogenase alpha-2 beta-2 (madA) gene, complete cds.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Claassen PA, et al. Tricarboxylic-acid and glyoxylate cycle enzyme activities in Thiobacillus versutus an isocitrate lyase negative organism. Arch. Microbiol. 145: 148-152, 1986.

Harrison AP Jr.. Genomic and physiological comparisons between heterotrophic thiobacilli and Acidiphilum cryptum, Thiobacillus versutus sp. nov., and Thiobacillus acidophilus nom. rev.. Int. J. Syst. Bacteriol. 33: 211-217, 1983.

Taylor BF, Hoare DS. New facultative Thiobacillus and a reevaluation of the heterotrophic potential of Thiobacillus novellus. J. Bacteriol. 100: 487-497, 1969. PubMed: 5344108

Lu WP, Kelly DP. Purification and some properties of two principal enzymes of the thiosulphate-oxidizing multi-enzyme system from Thiobacillus A2. J. Gen. Microbiol. 129: 3549-3565, 1983.

Katayama Y, et al. Paracoccus thiocyanatus sp. nov., a new species of thiocyanate-utilizing facultative chemolithotroph, and transfer of Thiobacillus versutus to the genus Paracoccus as Paracoccus versutus comb. nov. with emendation of the genus. Microbiology 141: 1469-1477, 1995. PubMed: 7545513

View All Curated Citations for this Product

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