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Acidithiobacillus ferrooxidans (Temple and Colmer) Kelly and Wood


Type strain.  Formerly Thiobacillus ferrooxidans.  Organism used in genome sequencing project.
Product category
Product type
Strain designation
NCIB 8455 [DSM 14882]
Type strain
Genome sequenced strain
Isolation source
Acid, bituminous coal mine effluent
Product format
Test tube
Storage conditions
See handling procedure
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications


Extremophile type
Sulfur oxidizer
Genome sequenced strain

Handling information

Handling procedure
  1. Incubate test tube cultures under above conditions upon receipt. It is helpful to incubate test tubes in a slanted position to increase gas exchange in broth. Transfer culture to fresh media within one week of arrival.
  2. Gently vortex the test-tube to dislodge the iron that has oxidized onto the glass. Aseptically withdraw approximately 1.0 mL of the broth culture and transfer into 5 mL of fresh broth.
  3. Incubate the broth either on a shaker or in a static and slanted position.
  4. Growth is evident within one week, when yellow-orange iron oxide deposits are observed.
  5. Transfer the culture approximately every two weeks.
Handling notes
The cells are motile and rod-shaped.
Additional information on this culture is available on the ATCC® web site at


Deposited as
Ferrobacillus ferrooxidans Leathen and Braley
Chain of custody
ATCC <-- NCIMB <-- P Sneath <-- Leathen
Type of isolate
Cross references
GenBank M11542 Thiobacillus ferrooxidans 5S ribosomal RNA.
GenBank M79404 16S rRNA sequence
GenBank M79405 16S rRNA sequence
GenBank M79406 16S rRNA sequence
GenBank X95324 T.ferrooxidans rustA gene.
GenBank AJ277640 Thiobacillus ferrooxidans tapA gene.
GenBank AH001792 Thiobacillus ferrooxidans 16S rRNA sequence.
GenBank AF129925 Thiobacillus ferrooxidans carboxysome operon, complete cds.
GenBank AF307091 Acidithiobacillus ferrooxidans ribulose bisphosphate carboxylase/oxygenase, large subunit (cbbL2), ribulose bisphosphate carboxylase/oxygenase, small subunit (cbbS2), and CbbQ (cbbQ) genes, complete cds.
GenBank CP001219 Acidithiobacillus ferrooxidans ATCC 23270, complete genome.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


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Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

Leathen WW, Braley SA. A new iron-oxidizing bacterium: Ferrobacillus ferrooxidans. Bacteriol. Proc. 1954: 44, 1954.

Biochem. Biophys. Res. Commun. 17: 603, 1964.

Can. J. Microbiol. 11: 23, 1965.

Braley SA Sr., et al. Ferrobacillus ferrooxidans: a chemosynthetic autotrophic Bacterium. J. Bacteriol. 72: 700-704, 1956. PubMed: 13376518

Silverman MP, Lundgren DG. Studies on the chemoautotrophic iron bacterium Ferrobacillus ferrooxidans. I. An improved medium and a harvesting procedure for securing high cell yields. J. Bacteriol. 77: 642-647, 1959. PubMed: 13654231

View All Curated Citations for this Product

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