Nitrosococcus oceani (Watson) Watson
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Transfer culture every four to six weeks, storing the fully-grown culture at 4°C. A 10% inoculum (10.0 mL per 100.0 mL fresh medium) is recommended. Cells may be preserved by freezing with a suitable cryoprotectant.
When starting ATCC® 19707™ in a flask of broth, ATCC typically observes pink medium changing to yellow within two weeks and growth that settles on the bottom of the flask. ATCC uses a T-25 with 10 mL of medium (laid flat) and incubate at 26°C. It is very important that the media needs to be fresh. The media should have a pink coloration and is sensitive to light. The media should be kept away from the light and refrigerated (this helps with keeping the media a little longer).
Within two weeks of starting the culture the media should have changed coloration to a pale yellow but the culture will not be dense enough to transfer. The culture should be fed K2CO3, until the color changes back to pink. You need to do this by a drop by drop basis so that you don't add too much.
The culture should be examined microscopically once the K2CO3 is beginning to be utilized. This culture does not get very dense and there will be only a few cells per field. The cells will appear as cocci in pairs and singles, motile by tuft of flagella.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
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If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Watson SW. Taxonomic considerations of the family Nitrobacteraceae Buchanan. Requests for opinions. Int. J. Syst. Bacteriol. 21: 254-270, 1971.
Truper HG, De Clari L. Taxonomic note: Necessary correction of specific epithets formed as substantives (nouns) "in apposition". Int. J. Syst. Bacteriol. 47: 908-909, 1997.
Hovanec TA, DeLong EF. Comparative analysis of nitrifying bacteria associated with freshwater and marine aquaria. Appl. Environ. Microbiol. 62: 2888-2896, 1996. PubMed: 8702281
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.