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Nitrosococcus oceani (Watson) Watson

19707

Type strain. Genome sequenced strain.
Product category
Bacteria
Product type
Extremophile
Strain designation
C-107
Type strain
Yes
Genome sequenced strain
Yes
Isolation source
Seawater, North Atlantic
Product format
Frozen
Storage conditions
-80°C or colder
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Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Preceptrol
No

Characteristics

Extremophile type
Halophile
Comments
Nomenclature
Genome sequenced strain

Handling information

Medium
Temperature
26°C
Atmosphere
Aerobic
Handling procedure
  1. Thaw frozen vial at room temperature and aseptically transfer vial contents to 10.0 mL of ATCC Medium #928 contained in an Erlenmeyer or tissue culture flask. Remove 1.0 mL and transfer to a second flask containing 10.0 mL.
  2. Transfer 0.1 mL to ATCC Medium #260 from primary flask and streak.
  3. Incubate flask and plate at 26°C for 7-14 days. Wrap the flask with a foil served as dark. When using a tissue culture flask, it should be laid down for incubation to promote air exchange.
  4. Growth is very slow but is evident by the change of color due to pH changes and microscopically with round cells found in pairs. After growth has been established the culture can be transferred (see notes). No growth observes on Medium #260.
Handling notes

Transfer culture every four to six weeks, storing the fully-grown culture at 4°C.  A 10% inoculum (10.0 mL per 100.0 mL fresh medium) is recommended.  Cells may be preserved by freezing with a suitable cryoprotectant.


When starting ATCC® 19707™ in a flask of broth, ATCC typically observes pink medium changing to yellow within two weeks and growth that settles on the bottom of the flask.  ATCC uses a T-25 with 10 mL of medium (laid flat) and incubate at 26°C.  It is very important that the media needs to be fresh.  The media should have a pink coloration and is sensitive to light.  The media should be kept away from the light and refrigerated (this helps with keeping the media a little longer). 


Within two weeks of starting the culture the media should have changed coloration to a pale yellow but the culture will not be dense enough to transfer.  The culture should be fed K2CO3, until the color changes back to pink.  You need to do this by a drop by drop basis so that you don't add too much. 


The culture should be examined microscopically once the K2CO3 is beginning to be utilized.  This culture does not get very dense and there will be only a few cells per field.  The cells will appear as cocci in pairs and singles, motile by tuft of flagella.


Additional information on this culture is available on the ATCC® web site at www.atcc.org.

History

Deposited as
Nitrosococcus oceanus (Watson) Watson
Depositors
SW Watson
Type of isolate
Environmental
Cross references
GenBank CP000127 Nitrosococcus oceani ATCC 19707, complete genome.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Watson SW. Taxonomic considerations of the family Nitrobacteraceae Buchanan. Requests for opinions. Int. J. Syst. Bacteriol. 21: 254-270, 1971.

Truper HG, De Clari L. Taxonomic note: Necessary correction of specific epithets formed as substantives (nouns) "in apposition". Int. J. Syst. Bacteriol. 47: 908-909, 1997.

Hovanec TA, DeLong EF. Comparative analysis of nitrifying bacteria associated with freshwater and marine aquaria. Appl. Environ. Microbiol. 62: 2888-2896, 1996. PubMed: 8702281

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

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