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Quantitative Genomic DNA from Acinetobacter baumannii strain 2208

19606DQ

Quantitative Genomic DNA from Acinetobacter baumannii strain 2208 can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load.
Product category
Bacteria
Product type
Molecular standard
Nucleic acid
Derived from
Acinetobacter baumannii 2208 [81, DSM 6974] (ATCC 19606)
Organism
Acinetobacter baumannii Bouvet and Grimont
Type strain
Yes
Genome sequenced strain
Yes
Applications
Assay development
Specification range
≥1 x 105 copies/μL
Volume
100 μL
Product format
Frozen
Storage conditions
-20°C or colder
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information

General

Specific applications
ATCC Genuine Nucleics can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load.

Characteristics

Comments
Genomic DNA isolated from a preparation of Acinetobacter baumannii strain 2208 (ATCC 19606). The source organism is also available through the ATCC catalog.

Handling information

Handling procedure
  1. Thaw the vial at room temperature and immediately place on ice. Avoid exposing the DNA to repeated freeze-thaw cycles as it may result in degradation.
  2. Gently mix the sample to ensure an even distribution of material.
  3. Briefly centrifuge the tube before opening to ensure all liquid is at the bottom.
Handling notes
Aliquoting is highly recommended to avoid multiple freeze-thaws.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Depositors
ATCC
Cross references
GenBank AX111482 Sequence 2215 from Patent WO0123604.
GenBank AX111503 Sequence 2236 from Patent WO0123604.
GenBank AX111523 Sequence 2256 from Patent WO0123604.
GenBank Z93435 Acinetobacter sp. 16S rRNA gene (strain ATCC 19606).
GenBank Z96926 Acinetobacter baumannii waaA gene, strain ATCC 19606.
GenBank AF100557 Acinetobacter baumannii DNA gyrase A (gyrA) gene, partial cds.
GenBank AB008684 Acinetobacter baumannii DNA for DNA gyrase subunit B, partial cds, strain CIP 70.34.
GenBank D73413 Acinetobacter baumannii gene for DNA gyrase subunit B protein, C terminal side, partial cds.
GenBank D73428 Acinetobacter baumannii gene for DNA gyrase subunit B protein, N terminal side, partial cds.
GenBank U60279 Acinetobacter baumannii 16S-23S ribosomal RNA intergenic spacer, including Ile-tRNA and Ala-tRNA genes.
GenBank AB001599 Acinetobacter baumannii DNA for L-2,4-diaminobutyrate:2-ketoglutarate 4-aminotransferase, complete cds.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Frequently Asked Questions

References

Curated Citations

Hugh R, Reese R. Designation of the type strain for Bacterium anitratum Schaub and Hauber 1948. Int. J. Syst. Bacteriol. 17: 245-254, 1967.

Schaub IG, Hauber FD. A biochemical and serological study of a group of identical unidentifiable gram negative bacilli from human sources. J. Bacteriol. 56: 379-385, 1948.

Hugh R, Reese R. A comparison of 120 strains of Bacterium anitratum Schaub and Hauber with the type strain of this species. Int. J. Syst. Bacteriol. 18: 207-229, 1968.

Bouvet PJ, Grimont PA. Taxonomy of the genus Acinetobacter with the recognition of Acinetobacter baumannii sp. nov., Acinetobacter haemolyticus sp. nov., Acinetobacter johnsonii sp. nov., and Acinetobacter junii sp. nov. and emended descriptions of Acinetobacter calcoaceticus and Acinetobacter lwoffii. Int J Syst Bacteriol 36: 228-240, 1986.

Listeria species -- Biochemical Identification Method (Vitek GPI and GNI+). Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 992.19.

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