Prevotella brevis Bryant et al.
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
2. Under anaerobic conditions, withdraw 0.5 ml of recommended broth from a single test tube (5 to 6 ml) and rehydrate the vial contents.
3. Aseptically transfer this aliquot back into the broth tube. A slant and a pre-reduced blood plate may also be inoculated with 0.1 ml each of the cell suspension. An aerobic blood plate may also be streaked to check for purity.
4. Incubate tubes and plate under anaerobic conditions at 37°C for 24 to 48 hours. Incubate blood plate aerobically at 37°C.
5. Within 48 to 72 hours, growth should be evident by turbidity in the broth. No growth should occur on the blood agar plate incubated aerobically.
Anaerobic conditions for transfer may be obtained by either of the following:
· Use of an anaerobic gas chamber, or
· Placement of test tubes under a gassing cannula system hooked to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
· Loose screw caps on test tubes in anaerobic chamber,
· Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
· Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
This organism requires rumen fluid for growth. It is also very sensitive to oxygen. All media must be completely reduced. Any oxygen exposure will cause the culture to become nonviable.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.
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Datta R. Process for the production of succinic acid by anaerobic fermentation. US Patent 5,143,833 dated Sep 1 1992
Bryant MP, et al. Bacteroides ruminicola n. sp. and Succinimonas amylolytica; the new genus and species; species of succinic acid-producing anaerobic bacteria of the bovine rumen. J. Bacteriol. 76: 15-23, 1958. PubMed: 13563384
Bladen HA, et al. A study of bacterial species of the rumen which produce ammonia from protein hydrolysate. Appl. Microbiol. 9: 175-180, 1961.
Bryant MP, Robinson IM. Some nutritional characteristics of predominant culturable ruminal bacteria. J. Bacteriol. 84: 605-614, 1962. PubMed: 14016429
Pittman KA, Bryant MP. Peptides and other nitrogen sources for growth of Bacteroides ruminicola. J. Bacteriol. 88: 401-410, 1964. PubMed: 14203357