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Brevundimonas diminuta (Leifson and Hugh) Segers et al.

19146

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Formerly Pseudomonas diminuta.  Used for sterility assurance and membrane filter testing.
Product category
Bacteria
Strain designation
FDA strain PCI 818 [CCUG 24715, DSM 1635, LMG 10743]
Type strain
No
Isolation source
Contaminant in culture of Bacillus cereus
Applications
Bioinformatics
Product format
Freeze-dried
Storage conditions
2°C to 8°C
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Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Pharmaceutical and Personal Care
Filtration sterilization control RefBritish Pharmacopoeia Commission Methods of sterilization. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVIII, 2003
Membrane filter testing RefASTM International Standard Test Method for Determining Bacterial Retention of Membrane Filters Utilized for Liquid Filtration. West Conshohocken, PA RefASTM International Standard Test Method for Retention Characteristics of 0.2-micrometer Membrane Filters Used in Routine Filtration Procedures for the Evaluation of Microbiological Water Quality. West Conshohocken, PA:ASTM International;ASTM Standard Test Method D 3862-80(Reapproved 2001). RefU.S. Pharmacopeia General Chapters: <1211> Sterilization and Sterility Assiramce of Compendial Articles. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011 Ref Aseptic processing of health care products-- Part 2: Filtration. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 13408-2:2003.
Sterility assurance RefU.S. Pharmacopeia General Chapters: <1211> Sterilization and Sterility Assiramce of Compendial Articles. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011 Ref Terminal Sterilization and Sterilization Indicators. Tokyo, Japan:Japanese Pharmacopoeia;JP JP14e.part II.15.
Testing Ref Physical Removal of Microbial Contamination Agent in Drinking Water. Washington, DC:Environmental Protection Agency;EPA EPA NSF 04/12/EPADWCTR. Ref Physical Removal of Microbial Contamination Agents in Drinking Water. Washington, DC:Environmental Protection Agency;EPA EPA NSF 04/13/EPADWCTR, 2004 Ref Physical Removal of Microbial Contamination Agents in Drinking Water. Washington, DC:Environmental Protection Agency;EPA EPA NSF 04/14/EPADWCTR, 2004
Preceptrol
No

Characteristics

Comments
Does not pass through a 0.2 micron membrane filter RefASTM International Standard Test Method for Retention Characteristics of 0.2-micrometer Membrane Filters Used in Routine Filtration Procedures for the Evaluation of Microbiological Water Quality. West Conshohocken, PA:ASTM International;ASTM Standard Test Method D 3862-80(Reapproved 2001).

Handling information

Medium
Temperature
30°C
Atmosphere
Aerobic
Handling procedure
  1. Open vial according to enclosed instructions or visit www.atcc.org for instructions.
  2. Rehydrate the entire pellet with approximately 0.5 mL of #2495 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #3 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
  3. Use several drops of the primary broth tube to inoculate a #3 plate and/or #3 agar slant.
  4. Incubate at 30°C for 48-72 hours.
Handling notes
Phosphate buffer is for rehydration and transfer only, not for growth.
Two colony types may be found in this strain if it is passed through Nutrient Broth (BD 234000).
To prevent proliferation of the second colony type, rehydrate the vial as above and maintain the culture by transferring from agar to agar.
Purified genomic DNA of this strain is available as ATCC® 19146D-5™.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Pseudomonas sp.
Depositors
FDA
Chain of custody
ATCC <-- FDA <-- FW Bowman and MP Calhoun
Type of isolate
Bacteria

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

ASTM International Standard Test Method for Determining Bacterial Retention of Membrane Filters Utilized for Liquid Filtration. West Conshohocken, PA

ASTM International Standard Test Method for Retention Characteristics of 0.2-micrometer Membrane Filters Used in Routine Filtration Procedures for the Evaluation of Microbiological Water Quality. West Conshohocken, PA:ASTM International;ASTM Standard Test Method D 3862-80(Reapproved 2001).

Segers P, et al. Classification of Pseudomonas diminuta Leifson and Hugh 1954 and Pseudomonas vesicularis Busing, Doll, and Freytag 1953 in Brevundimonas gen. nov. as Brevundimonas diminuta comb. nov. and Brevundimonas vesicularis comb. nov., respectively. Int. J. Syst. Bacteriol. 44: 499-510, 1994. PubMed: 8068543

British Pharmacopoeia Commission Methods of sterilization. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVIII, 2003

U.S. Pharmacopeia General Chapters: <1211> Sterilization and Sterility Assiramce of Compendial Articles. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011

View All Curated Citations for this Product

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