Thermobispora bispora (Henssen) Wang et al.
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2. Using a single tube of #1877 broth (5 to 6 ml), withdraw approximately 0.5 ml to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the pellet.
3. Aseptically transfer this aliquot back into the broth tube and mix well.
4. Use several drops of the suspension to inoculate a second tube of broth, a #527 slant and/or plate.
5. Incubate all tubes and plate at 50oC for 5 to 7 days.
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Lechevalier HA. Priority of the generic name Microbispora over Waksmania and Thermopolyspora. Int. Bull. Bacteriol. Nomencl. Taxon. 15: 139-142, 1965.
Henssen A. Morphology and system of thermophilic actinomycetes. Arch. Mikrobiol. 26: 373-414, 1957. PubMed: 13435805
Henssen A, Schnepf E. On the knowledge of thermophilic actinomycetes. Arch. Mikrobiol. 57: 214-231, 1967. PubMed: 5600778
Wang Y, et al. The actinomycete Thermobispora bispora contains two distinct types of transcriptionally active 16S rRNA genes. J. Bacteriol. 179: 3270-3276, 1997. PubMed: 9150223